LEY 26872 PDF

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View Ideas submitted by the community. Received Mar 3; Accepted Apr All mice were housed in a specific pathogen-free barrier facility and were screened regularly for pathogens.

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Ley K, Tedder T F. Automatic update in Gaboury J P, Kubes P. Cumulative Upper and conventional histograms of the distribution of leukocyte rolling velocities in adhesion molecule-deficient mice. Thus, the EGF-like domain of P-selectin may play a direct role in ligand recognition and leukocyte adhesion mediated by P-selectin, with the lectin plus EGF-like domains collectively forming a functional ligand recognition unit.

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The cremaster muscle was prepared for intravital microscopy as described 12 The difference between the end points of the traveled distance was measured either on the monitor with a caliper or by using a custom digital image processing system Microvessel diameter and individual rolling leukocyte velocity were measured by using a digital image processing system.

Open in a separate window. Leukocyte rolling flux fraction is defined as the flux of rolling leukocytes as a percent of total leukocyte flux. However, the current in vivo results Fig.

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Find all citations in this journal default. Neutrophils normally represent a minority of the circulating leukocytes, but most rolling leukocytes oey these conditions are neutrophils The recruitment of leukocytes into sites of acute and chronic inflammation involves leukocyte interactions with vascular endothelium under conditions of shear flow.

Rolling leukocyte flux was determined by counting the number of leukocytes passing through each venule as ,ey 932 ICAMmediated decreases in leukocyte rolling velocities are likely to increase the frequency of firm adhesions between leukocytes and endothelial cells in vivo 37 The in vivo relevance of these observations is unknown.

Circulating leukocyte counts are factored into the equation used for determining rolling flux fractions, thus controlling for higher or lower systemic counts. Acknowledgments We thank Dr.

Login to enter a peer comment or grade. Because rolling at these time points is predominantly mediated by L-selectin with a contribution by P-selectin 933these results suggest that ICAM-1 expression also is required for optimal L-selectin-mediated rolling. The properties of 65— rolling leukocytes were assessed in 13—73 venules of each mouse line at time points between 10 and min after exteriorization of the cremaster muscle.

Although integrin function predominates during firm adhesive interactions between cells, the selectins also may contribute to this process Of primary importance are two findings.

To further define the cascade of interactions among families of adhesion molecules during leukocyte—endothelial cell interactions in vivomice deficient in both L-selectin and ICAM-1 were generated.

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Return to KudoZ list. Nature London ; The KudoZ network provides a framework for translators and others to leg each other with translations or explanations of terms and short phrases. Exchange of only the lectin domains between L- and P-selectin conferred the adhesive and ligand recognition functions of the lectin domain of the parent molecule. Post Your ideas for ProZ.

Studies 22 under in vitro conditions of shear flow have suggested that Ly ligands and ICAM-1 are engaged in series by leukocytes, rather than in parallel.

The generation 268872 mice deficient in both ICAM-1 and L-selectin has provided a powerful tool to further unravel the complex interactions that occur between the selectins and members of the Ig superfamily during leukocyte interactions with endothelial cells at sites of inflammation.

National Center for Biotechnology InformationU. L-selectin CD62L is expressed constitutively by key leukocytes whereas P-selectin CD62P and E-selectin CD62E are expressed by activated endothelial cells and primarily mediate neutrophil and monocyte rolling 4. These results demonstrate that ICAM-1 expression influences selectin-mediated rolling of leukocytes in vivoin addition to its well established roles in firm adhesion and transmigration of leukocytes at sites of inflammation